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dip100 quantikine immunoassay  (R&D Systems)


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    R&D Systems dip100 quantikine immunoassay
    Dip100 Quantikine Immunoassay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cxcl10+quantikine+elisa+kit/Human+CXCL10%2FIP-10+Quantikine+ELISA+Kit/us12601747-328-9-26
    Average 96 stars, based on 197 article reviews
    dip100 quantikine immunoassay - by Bioz Stars, 2026-11
    96/100 stars

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    Recombinant:

    Article Title: Inflammatory Markers of the Systemic Capillary Leak Syndrome (Clarkson Disease)
    Article Snippet: Qdot 605-conjugated anti-CD3 and Live/Dead fixable violet dead stain kit were from Life Technologies. .. Recombinant human TNFα and IFNγ, and CXCL10 Quantikine ELISA kit were purchased from R & D Systems. .. Serum cytokines were measured using a Bio-plex Pro human cytokine 27-plex and a customized human cytokine 9-plex assay kit purchased from Bio-Rad according to the manufacturer’s instructions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inflammatory Markers of the Systemic Capillary Leak Syndrome (Clarkson Disease)
    Article Snippet: Qdot 605-conjugated anti-CD3 and Live/Dead fixable violet dead stain kit were from Life Technologies. .. Recombinant human TNFα and IFNγ, and CXCL10 Quantikine ELISA kit were purchased from R & D Systems. .. Serum cytokines were measured using a Bio-plex Pro human cytokine 27-plex and a customized human cytokine 9-plex assay kit purchased from Bio-Rad according to the manufacturer’s instructions.

    Article Title: Nab‐paclitaxel interrupts cancer‐stromal interaction through C‐X‐C motif chemokine 10‐mediated interleukin‐6 downregulation in vitro
    Article Snippet: .. Level of IL‐6 and CXCL10 was determined using IL‐6 and CXCL10 Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol. .. Absorbance at 450 nm was measured using a plate reader (SpectraMax i3; Molecular Devices, Tokyo, Japan) at a correction wavelength of 540 nm.

    other:

    Article Title: CXCL9 and CXCL10 Induce Expression of Nociceptive Ion Channels in Primary Sensory Neurons in Models of HIV-Associated Distal Sensory Polyneuropathy
    Article Snippet: MDMs were differentiated for 7 days and subsequently treated with 10 ng/mL LPS (Thermofisher) or 10 ng/mL IFNγ (R&D Systems) for 24 h. Following treatment, conditioned media was collected for XL cytokine arrays and treatment of iPSC-PSNs.

    Article Title: CXCL9 and CXCL10 Induce Expression of Nociceptive Ion Channels in Primary Sensory Neurons in Models of HIV-Associated Distal Sensory Polyneuropathy
    Article Snippet: Cells were permeabilized with PBST with 0.1% Triton-X100 (Sigma-Aldrich) and blocked using PBST with 2% BSA (Thermofisher) and 5% FBS (Gibco).



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    The differences in the expression and clinical correlation of CXCL10 were evaluated in breast cancer patients. ( A ) The role of CXCL10 in breast cancer was predicted with the CanerSEA database. ( B ) Differences in the expression of CXCL10 in different types of breast cancer were analyzed with the TIMER2.0 database. ( C ) Based on GEPIA2 breast cancer data, a volcano map was drawn with bioinformatics to determine the expression level of CXCL10. ( D ) The relationship between CXCL10 and the clinical prognosis of breast cancer patients was analyzed with the TIMER2.0 database. ( E ) The expression level of CXCL10 in adjacent and cancerous tissues was detected by immunohistochemistry. ( F ) The expression differences in different clinical stages were analyzed with the GEPIA2 database. (*** p < 0.001)

    Journal: Clinical and Experimental Medicine

    Article Title: CXCL12-mediated T cell infiltration drives breast cancer metastasis

    doi: 10.1007/s10238-026-02126-2

    Figure Lengend Snippet: The differences in the expression and clinical correlation of CXCL10 were evaluated in breast cancer patients. ( A ) The role of CXCL10 in breast cancer was predicted with the CanerSEA database. ( B ) Differences in the expression of CXCL10 in different types of breast cancer were analyzed with the TIMER2.0 database. ( C ) Based on GEPIA2 breast cancer data, a volcano map was drawn with bioinformatics to determine the expression level of CXCL10. ( D ) The relationship between CXCL10 and the clinical prognosis of breast cancer patients was analyzed with the TIMER2.0 database. ( E ) The expression level of CXCL10 in adjacent and cancerous tissues was detected by immunohistochemistry. ( F ) The expression differences in different clinical stages were analyzed with the GEPIA2 database. (*** p < 0.001)

    Article Snippet: After 24 h of incubation, the culture supernatants were analyzed with a human CXCL10 ELISA kit (R&D Systems, QK266).

    Techniques: Expressing, Immunohistochemistry

    The composition of cells in the breast cancer tissue microenvironment, interactions between cells, and differences in CXCL10 expression between breast cancer cells and T cells were analyzed with the single-cell sequencing data. ( A - B ) The composition of the main cells in the microenvironment of breast cancer tissue was analyzed with the NGDC database. ( C ) The interaction relationships between various types of cells were analyzed. ( D ) The CXCL10 expression levels were analyzed in various types of cells

    Journal: Clinical and Experimental Medicine

    Article Title: CXCL12-mediated T cell infiltration drives breast cancer metastasis

    doi: 10.1007/s10238-026-02126-2

    Figure Lengend Snippet: The composition of cells in the breast cancer tissue microenvironment, interactions between cells, and differences in CXCL10 expression between breast cancer cells and T cells were analyzed with the single-cell sequencing data. ( A - B ) The composition of the main cells in the microenvironment of breast cancer tissue was analyzed with the NGDC database. ( C ) The interaction relationships between various types of cells were analyzed. ( D ) The CXCL10 expression levels were analyzed in various types of cells

    Article Snippet: After 24 h of incubation, the culture supernatants were analyzed with a human CXCL10 ELISA kit (R&D Systems, QK266).

    Techniques: Expressing, Single Cell, Sequencing

    Correlation and differential expression analyses of CXCL10-interacting molecules were performed in BC. ( A ) CXCL10-interacting molecules were analyzed with the String database. ( B - E ) The correlations between CXCL10 and CCL13, CXCL6, PF4V1, and CXCL12 were analyzed with the GEPIA2 database. ( F - I ) Differences in the expression of CCL13, CXCL6, PF4V1 and CXCL12 in breast cancer were analyzed with the GEPIA2 database. ( J ) The differential expression of CXCL10 and CXCL12 in breast epithelial cells and breast cancer cells was detected by western blotting. (* p < 0.05)

    Journal: Clinical and Experimental Medicine

    Article Title: CXCL12-mediated T cell infiltration drives breast cancer metastasis

    doi: 10.1007/s10238-026-02126-2

    Figure Lengend Snippet: Correlation and differential expression analyses of CXCL10-interacting molecules were performed in BC. ( A ) CXCL10-interacting molecules were analyzed with the String database. ( B - E ) The correlations between CXCL10 and CCL13, CXCL6, PF4V1, and CXCL12 were analyzed with the GEPIA2 database. ( F - I ) Differences in the expression of CCL13, CXCL6, PF4V1 and CXCL12 in breast cancer were analyzed with the GEPIA2 database. ( J ) The differential expression of CXCL10 and CXCL12 in breast epithelial cells and breast cancer cells was detected by western blotting. (* p < 0.05)

    Article Snippet: After 24 h of incubation, the culture supernatants were analyzed with a human CXCL10 ELISA kit (R&D Systems, QK266).

    Techniques: Quantitative Proteomics, Expressing, Western Blot

    The differences in the expression and clinical correlation of CXCL12 were evaluated in BC patients. ( A ) Differences in CXCL12 expression in different types of breast cancer were analyzed with the TIMER2.0 database. ( B ) Based on GEPIA2 breast cancer data, a volcano map was drawn by bioinformatics to determine the expression of CXCL12. ( C ) Differences in the expression of CXCL12 in different clinical stages were analyzed with the GEPIA2 database. ( D ) The relationship between CXCL12 and the prognosis of breast cancer patients was analyzed with the TIMER2.0 database. ( E - F ) The expression of CXCL12 in different types of cells was analyzed with single-cell sequencing from the NGDC database. ( G ) The expression level of CXCL10 in adjacent and cancerous tissues was detected by immunohistochemistry. (*** p < 0.001)

    Journal: Clinical and Experimental Medicine

    Article Title: CXCL12-mediated T cell infiltration drives breast cancer metastasis

    doi: 10.1007/s10238-026-02126-2

    Figure Lengend Snippet: The differences in the expression and clinical correlation of CXCL12 were evaluated in BC patients. ( A ) Differences in CXCL12 expression in different types of breast cancer were analyzed with the TIMER2.0 database. ( B ) Based on GEPIA2 breast cancer data, a volcano map was drawn by bioinformatics to determine the expression of CXCL12. ( C ) Differences in the expression of CXCL12 in different clinical stages were analyzed with the GEPIA2 database. ( D ) The relationship between CXCL12 and the prognosis of breast cancer patients was analyzed with the TIMER2.0 database. ( E - F ) The expression of CXCL12 in different types of cells was analyzed with single-cell sequencing from the NGDC database. ( G ) The expression level of CXCL10 in adjacent and cancerous tissues was detected by immunohistochemistry. (*** p < 0.001)

    Article Snippet: After 24 h of incubation, the culture supernatants were analyzed with a human CXCL10 ELISA kit (R&D Systems, QK266).

    Techniques: Expressing, Single Cell, Sequencing, Immunohistochemistry

    The impact of CXCL12 on breast cancer and its correlation with CXCL10 were evaluated. ( A ) CXCL12 knockdown efficiency was detected by Q-PCR. ( B - C ) The impact of CXCL12 knockdown on cell invasion ability was detected by transwell assays. ( D - E ) The effect of CXCL12 knockdown on cell migration ability was detected by scratch assays. ( F ) The effect of CXCL12 knockdown on CXCL10 expression was detected by Q-PCR. ( G ) The effect of CXCL12 knockdown on CXCL10 secretion levels was detected by ELISA. (*** p < 0.001)

    Journal: Clinical and Experimental Medicine

    Article Title: CXCL12-mediated T cell infiltration drives breast cancer metastasis

    doi: 10.1007/s10238-026-02126-2

    Figure Lengend Snippet: The impact of CXCL12 on breast cancer and its correlation with CXCL10 were evaluated. ( A ) CXCL12 knockdown efficiency was detected by Q-PCR. ( B - C ) The impact of CXCL12 knockdown on cell invasion ability was detected by transwell assays. ( D - E ) The effect of CXCL12 knockdown on cell migration ability was detected by scratch assays. ( F ) The effect of CXCL12 knockdown on CXCL10 expression was detected by Q-PCR. ( G ) The effect of CXCL12 knockdown on CXCL10 secretion levels was detected by ELISA. (*** p < 0.001)

    Article Snippet: After 24 h of incubation, the culture supernatants were analyzed with a human CXCL10 ELISA kit (R&D Systems, QK266).

    Techniques: Knockdown, Migration, Expressing, Enzyme-linked Immunosorbent Assay

    Correlations between CXCL12 and CXCL10 and CD4 + T and CD8 + T cells in the inflammatory microenvironment of breast cancer patients were evaluated, and correlations with the clinical prognosis of breast cancer patients were evaluated. ( A ) The role of CXCL10 in breast cancer was predicted with the CanerSEA database. ( B ) The expression levels of CXCL10 and CXCL12 in different types of T lymphocytes were analyzed in different types of breast cancer with the TIMER2.0 database. ( C - F ) The clinical prognosis under different conditions was analyzed with the TIMER2.0 database. ( G ) CD4 + T cells and CD8 + T cells in breast cancer tissues were analyzed by immunofluorescence

    Journal: Clinical and Experimental Medicine

    Article Title: CXCL12-mediated T cell infiltration drives breast cancer metastasis

    doi: 10.1007/s10238-026-02126-2

    Figure Lengend Snippet: Correlations between CXCL12 and CXCL10 and CD4 + T and CD8 + T cells in the inflammatory microenvironment of breast cancer patients were evaluated, and correlations with the clinical prognosis of breast cancer patients were evaluated. ( A ) The role of CXCL10 in breast cancer was predicted with the CanerSEA database. ( B ) The expression levels of CXCL10 and CXCL12 in different types of T lymphocytes were analyzed in different types of breast cancer with the TIMER2.0 database. ( C - F ) The clinical prognosis under different conditions was analyzed with the TIMER2.0 database. ( G ) CD4 + T cells and CD8 + T cells in breast cancer tissues were analyzed by immunofluorescence

    Article Snippet: After 24 h of incubation, the culture supernatants were analyzed with a human CXCL10 ELISA kit (R&D Systems, QK266).

    Techniques: Expressing, Immunofluorescence